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Original Research Article | OPEN ACCESS

In vitro anti-tumor activity in SGC-7901 human gastric cancer cells treated with dandelion extract

Hui Han1, Geng Zhen Chen1 , Sheng Kang Zhou2, Rui Ru Xu1, Cheng Liang Wu1

1Department of General Surgery, The Second Affiliated Hospital of Shantou Medical College, Shantou; 2Department of Gastrointestinal Surgery, Tai Zhou Hospital, Tai Zhou City, People's Republic of China.

For correspondence:-  Geng Chen   Email: hjbmopvb@sina.com

Accepted: 17 October 2017        Published: 31 January 2018

Citation: Han H, Chen GZ, Zhou SK, Xu RR, Wu CL. In vitro anti-tumor activity in SGC-7901 human gastric cancer cells treated with dandelion extract. Trop J Pharm Res 2018; 17(1):65-70 doi: 10.4314/tjpr.v17i1.10

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the mechanisms of cytotoxicity of a dandelion extract against human gastric cancer cell line SGC-7901 cells.
Methods: The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, flow cytometry, and transwell assays were used to investigate the effects of a dandelion extract on cell proliferation, levels of apoptosis, and cell migration, respectively. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assessed the effects of a dandelion extract on the expression levels of genes regulating proliferation and apoptosis.
Results: Dandelion extract exerted strong cytotoxic effects on the cancer cells. After exposure, apoptotic cells increased and cell migration was reduced. RT-qPCR assay revealed that dandelion extract significantly increased anti-proliferative and pro-apoptotic gene expression, including phosphate and tensin homology deleted on chromosome ten (Pten) and Bcl-2 Associated X protein (Bax) mRNA in the gastric cancer cells. The results also indicate that there was decreased pro-proliferative and anti-apoptotic gene expression (i.e., extracellular signal-regulated kinase (Erk), Survivin, and B-cell lymphoma 2 (Bcl2) mRNA).
Conclusion: The results suggest that dandelion extract is a potent gastric cancer cell proliferation, survival, and migration inhibitor with potential pharmaceutical applications for the prevention of gastric cancer
 

Keywords: Dandelion extract, gastric cancer, cytotoxic effect, migration inhibition

Impact Factor
Thompson Reuters (ISI): 0.523 (2021)
H-5 index (Google Scholar): 39 (2021)

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